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FLUORESCENT IMAGING
The principal equipment of our core facility is a Zeiss Axio Observer Z.1 inverted fluorescence microscope, which represents the highest level of fluorescence microscopes. Its various polarizers allow not only phase-contrast, but the so-called DIC (Differential Interference Contrast) imaging. Detection of most of the fluorescent proteins (GFP, YFP and CFP) and a number of fluorescent dyes (DAPI, FITC, Cy3, Rhodamine, TRITC) is possible with the use of its filters. It has a self-adjusting HBO high pressure mercury lamp, which is gentle to the cells and dyes. The fully-motorized filter changer is required for rapid multi-channel imaging. The microscope is equipped with various types of lenses, 5x - 100x range, that are moved completely motorizedly. Its stage is also fully motorized allowing multi-dimensional imaging. The microscope is equipped with a high-resolution color camera and a high-sensitivity monochrome camera that are able to do fluorescence imaging in video (time lapse) mode.  LASER MICRODISSECTION
 The Zeiss Axio Observer Z.1 inverted fluorescence microscope is also equipped with PALM MicroBeam laser microdissection module. This module enables isolation of microscopic particles (eg, portions of tissue, individual cells, chromosomes) from fixed or live tissue samples or cell culture (1 µm to 200 µm thickness). The microdissection procedure, both the cutting and the moving, catapulting of the cut sample is performed by UV laser light (LMPC = Laser Microdissection and Pressure Catapulting). This advanced contact-free technology ensures that the isolated sample is not damaged during the process, remaines free from contamination and degradation. Collection of isolated samples is done by CapMover. The fully automated system is highly precise, able to isolate more than one area, and to perform three-dimensional cuts. The microdissection can be combined with fluorescent imaging, this way a number of experimental combinations are possible, such as selection of transfected adherent cells - without FACS - simply from the culture dish.
We also have a Micron HM 650V vibrotome for preparing microscopic sections. 
Fields of application
Laser microdissection can be used in cell biological, genetic / genomic and molecular biological research, as well as for pathological and forensic examinations and mineralogical analysis.
Source materials: - pathological, histological samples
- cryopreserved tissues
- formalin-fixed paraffin-embedded sections
- living cells or tissues
- stained or fluorescently marked cells
- chromosome preparations
- plant samples
- forensic samples
Possible subsequent analyses:
- DNA isolation: PCR, traditional or next generation sequencing, mutational analysis, SNP, genotyping, FISH
- RNA isolation: RT-PCR, expression analysis with sequencing or microarray
- protein extract: immunoblot, 2D gel analysis, proteomic analysis
- living cells: recultivation, generation of cell lines, isolation of stem cells
Contact person:
Dr. Éva Bálint
Research group leader
BAYGEN Institute
6726 Szeged
Derkovits fasor 2.
balinte[at]baygen.hu
Phone: +36 62 566 444
Fax:+36 62 566 440
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> Imaging & Microdissection 
